Main sol. 1155
Internal Solution-ID: S2319
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 27.70 | g | 27.7 | 473.99 | |
| MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
| MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 | |
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| CaCl2 x 2 H2O | 0.75 | g | 0.75 | 5.102 | |
| KCl | 0.66 | g | 0.66 | 8.853 | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| NaBr | 0.10 | g | 0.1 | 0.972 | |
| H3BO3 | 30.00 | mg | 0.03 | 0.485 | |
| SrCl2 x 6 H2O | 15.00 | mg | 0.015 | 0.056 | |
|
KJ
(1 mg/ml) |
50.00 | µl | - | - | |
| Elemental sulfur | 10.00 | g | 10 | - | |
| Double distilled water | 1000.00 | ml | - | - | |
|
Na2S
, used for reduction of the medium
(2.5 %) |
20.00 | ml | 0.5 | 6.324 | |
| Yeast extract | 0.04 | % | 0.4 | - | |
| 1 Reduction of the medium by addition of 20 ml Na2S (2.5%); then adjust pH to 5.5 with sulfuric acid. | |||||
| 2 Gas phase: H2/CO2=80/20 (250 kPa). | |||||
| 3 After autoclaving add 0.04% yeast extract. | |||||
| 4 Incubation at 90°C for 1-2 days. | |||||
Media with this solution
| 1155 | SME MEDIUM |