Main sol. 1157
Internal Solution-ID: S2323
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 2.0 | g | 2 | - | |
| Yeast extract | 2.0 | g | 2 | - | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 After autoclaving and cooling add: | |||||
|
Riboflavin
(0.2 mg/ml, filter-sterilized) |
5.0 | ml | - | - | |
|
Glucose
(autoclaved, 50%) |
2.0 | ml | 1 | 5.551 | |
|
MgSO4
(autoclaved, 20%) |
1.0 | ml | 0.2 | 1.662 | |
|
CaCl2
(autoclaved, 10%) |
1.0 | ml | 0.1 | 0.901 | |
| 2 Final pH 7.0 | |||||
Media with this solution
| 1157 | PYEM MEDIUM |