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Main sol. 1166a

Internal Solution-ID: S2338

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.00 g 5.982 102.362
K2HPO4 1.00 g 0.997 5.724
Na2SO4 2.80 g 2.792 19.654
NaHCO3 8.00 g 7.976 94.945
Na2CO3 22.00 g 21.934 206.95
NH4Cl 0.20 g 0.199 3.728
MgCl2 x 6 H2O 0.20 g 0.199 0.981
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.05 g 0.05 -
Na-pyruvate 2.20 g 2.193 19.932
Na2HAsO4 x 7 H2O 6.20 g 6.181 19.812
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.24 g 0.239 0.996
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, hydrogenphosphate and sodium sulfate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve hydrogencarbonate and carbonate, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, pyruvate, arsenate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of vitamins, pyruvate and arsenate should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 10.
1166a DESULFURIBACILLUS MEDIUM (ARSENATE)