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Main sol. 1167

Internal Solution-ID: S2339

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nutrient broth 1.0 g 1 -
Soy peptone
(e.g. Bacto Soytone)
1.0 g 1 -
Yeast extract 1.0 g 1 -
Tap water 1000.0 ml - -
1 The following inorganic solution may substitute for tap water:
MgSO4 x 7 H2O 75.0 mg 0.075 0.304
Ca(NO3)2 x 4 H2O 43.0 mg 0.043 0.182
NaHCO3 16.0 mg 0.016 0.19
KCl 5.0 mg 0.005 0.067
K2HPO4 x 3 H2O 3.7 mg 0.004 0.021
Distilled water 1000.0 ml - -
Trace element solution SL-4 0.1 ml - -
2 Adjust to pH 7.2.
1167 NSY-MEDIUM FOR POLYNUCLEOBACTER