Solid media
Internal Solution-ID: S2503
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
MES
(2-Morpholinoethanesulfonic acid) |
3.9 | g | 3.9 | 19.975 | |
|
MgSO4 x 7 H2O
(20 mM) |
20.0 | ml | 0.099 | 0.4 | |
|
CaCl2 x 2 H2O
(30 mM) |
20.0 | ml | 0.088 | 0.6 | |
|
(NH4)2HPO4
(20 mM) |
20.0 | ml | 0.053 | 0.4 | |
| Selenite-tungstate solution | 2.0 | ml | - | - | |
| Trace element solution SL-10 | 2.0 | ml | - | - | |
| Distilled water | 920.0 | ml | - | - | |
| 1 Adjust to pH 5.5 with NaOH/KOH solution (see below). | |||||
| 2 After autoclaving, keep at about 60°C, then add to each 250 ml lot: | |||||
| Vitamin solution | 1.5 | ml | - | - | |
|
Glucose
(filter-sterilized, 0.2M) |
10.0 | ml | 0.36 | 2 | |
|
Agar
(autoclaved, kept about 60°C, 3%, washed) |
250.0 | ml | 7.5 | 22.3 | |
| 3 Autoclave in 250ml lots, with a magnetic stirring bar in each bottle. | |||||
Media with this solution
| 1266 | MEDIUM VL55 |