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Solution 1

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Internal Solution-ID: S2514

Compound Amount Unit Conc. [g/L] Conc. [mM]
MES
(2-Morpholinoethanesulfonic acid) 		1.95 g 3.963 20.3
20 mM MgSO4 x 7 H2O 10.00 ml 0.049 0.2
30 mM CaCl2 x 2 H2O 10.00 ml 0.044 0.3
20mM (NH4)2HPO4 10.00 ml 0.026 0.2
Selenite-tungstate solution 1.00 ml - -
Trace element solution SL-10 1.00 ml - -
Distilled water 460.00 ml - -
1 Adjust to pH 5.5 with NaOH/KOH solution (see below).
1266a MEDIUM GY VL55