Main sol. 1279
Internal Solution-ID: S2559
Equipment needed: Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Brucella Broth
(Becton Dickinson) |
2.80 | g | 35 | - | |
| Demineralized water | 80.00 | ml | - | - | |
| 1 Add aseptically to each tube: | |||||
|
Fetal bovine serum
(heat inactivated) |
2.00 | ml | - | - | |
|
Vitox
(Oxoid) |
0.06 | ml | - | - | |
|
Skirrow supplement
(Oxoid) |
0.02 | ml | - | - | |
|
Amphotericin
(10 mg/ml) |
0.02 | ml | - | - | |
| 2 Adjust with HCl to pH 5.0, make anoxic under a stream of N2/CO2, distribute in Hungate tubes to 8 ml and autoclave. | |||||
| 3 Add 1 ml of sterile air to each tube. | |||||
Media with this solution
| 1279 | HELICOBACTER MEDIUM |