Main sol. 1315
Internal Solution-ID: S2631
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KNO3 | 0.85 | g | 0.85 | 8.407 | |
| NaHCO3 | 2.52 | g | 2.52 | 29.998 | |
| CaCl2 | 0.01 | g | 0.01 | 0.09 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| MnSO4 | 0.01 | g | 0.01 | 0.066 | |
| NH4Cl | 0.30 | g | 0.3 | 5.609 | |
| NaCl | 0.05 | g | 0.05 | 0.856 | |
| Selenite and tungstate solution | 2.00 | ml | - | - | |
| Distilled water | 884.00 | ml | - | - | |
| Phosphate solution | 100.00 | ml | - | - | |
| Acetate solution 98.0 g/L | 10.00 | ml | - | - | |
| Vitamin solution | 5.00 | ml | - | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| 1 Distribute to gas tight vessels, gas with 90% N2 +10% CO2 to achieve anaerobic conditions and autoclave. After cooling, add the phosphate solution, substrate solution, vitamin solution and trace element solution. After mixing, pH should be 7.1 - 7.5. | |||||
Media with this solution
| 1315 | ANAEROBIC THAUERA MEDIUM |