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Main sol. 150

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Internal Solution-ID: S264

Equipment needed: Autoclave Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 3 22.703
K2HPO4 x 3 H2O 0.50 g 0.5 2.871
MgSO4 x 7 H2O 0.50 g 0.5 2.029
KCl 0.10 g 0.1 1.341
Ca(NO3)2 x 4 H2O 0.02 g 0.02 0.085
Yeast extract 0.20 g 0.2 -
Sulfur
(powdered) 		10.00 g 10 311.818
Distilled water 1000.00 ml - -
1 Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. For sterilization of the sulfur place it in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium.
150 ACIDIANUS BRIERLEYI MEDIUM