Main sol. 1340
Internal Solution-ID: S2688
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| MgCl2 x 7 H2O | 0.5 | g | 0.5 | 5.252 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| NaCl | 200.0 | g | 200 | 3422.31 | |
| KNO3 | 2.0 | g | 2 | 19.782 | |
| Trace Elements Solution | 10.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the liquid medium. Adjust to pH 8.3 with 10 M KOH solution. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions: | |||||
|
NaHCO3
(10% w/v) |
20.0 | ml/l | 2 | 23.808 | |
|
Na2S x 9 H2O
(2% w/v) |
20.0 | ml/l | 0.4 | 1.665 | |
|
MgCl2 x 6 H2O
(10% w/v) |
20.0 | ml/l | 2 | 9.838 | |
|
Glucose
(1M) |
20.0 | ml/l | 3.603 | 20 | |
|
Yeast extract
(10% w/v) |
10.0 | ml/l | 1 | - | |
|
Cysteine-HCl
(10% w/v) |
5.0 | ml/l | 0.5 | 3.172 | |
Media with this solution
| 1340 | SEHEL FOR HALANAEROBIUM |