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Main sol. 1361

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Internal Solution-ID: S2742

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.14 g 0.14 1.029
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.15 g 0.15 1.02
NH4Cl 0.54 g 0.54 10.095
Trace elements Solution 1.00 ml - -
Glucose 0.18 g 0.18 0.999
Yeast extract 0.20 g 0.2 -
Vitamin solution 1.00 ml - -
Resazurin
(0.1 v/v) 		0.50 ml - -
NaHCO3 2.50 g 2.5 29.759
Na2S x 9 H2O 0.30 g 0.3 1.249
Cysteine HCl 0.30 g 0.3 2.476
Distilled water 1000.00 ml - -
1 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v). Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v) and add the NaHCO3. The final pH should be 7.0. Autoclave the medium and add from sterile stock solutions yeast extract, glucose, cysteine hydrochloride, vitamin solution and Na2S x 9 H2O.The final pH should be 7.0.
1361 PLANCTOMYCETALES BACTERIUM MEDIUM