Main sol. 163
Internal Solution-ID: S279
Equipment needed: Autoclave
Gassing station
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 980 | ml | - | - | |
| Solution B | 10 | ml | - | - | |
| Solution C | 10 | ml | - | - | |
| 1 Bring solution A to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary. | |||||
Media with this solution
| 163 | HALODESULFOVIBRIO (POSTGATE) MEDIUM |