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DSMZ

DSMZ 163 . HALODESULFOVIBRIO (POSTGATE) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 163 #

ml
Equipment needed: Autoclave Gassing station Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 980 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
1 Bring solution A to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.

Solution A #

980
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.0 g 25.51 436.52
K2HPO4 0.5 g 0.51 2.929
NH4Cl 1.0 g 1.02 19.077
Na2SO4 1.0 g 1.02 7.184
CaCl2 x 2 H2O 0.1 g 0.102 0.694
MgSO4 x 7 H2O 2.0 g 2.041 8.28
Na-DL-lactate 2.0 g 2.041 18.212
Yeast extract 1.0 g 1.02 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 -
Distilled water 980.0 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.5 g 50 179.846
Distilled water 10.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-thioglycolate 0.1 g 10 87.643
Ascorbic acid 0.1 g 10 56.78
Distilled water 10.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 15
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.79
Na-DL-lactate 2 17.848
MgSO4 x 7 H2O 2 8.114
NH4Cl 1 18.695
Na2SO4 1 7.04
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Cultivation metadata from 15 strains BacDive

halophily moderately halophilic 1 strain
pH 6 - 8 2 strains
pH 8 - 10 1 strain
pH 4 - 6 1 strain
phyla 1 15 strains
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