Main sol. 1383
Internal Solution-ID: S2803
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
MES
(2-Morpholinoethanesulfonic acid) |
1.95 | g | 2.063 | 10.569 | |
|
MgSO4 x 7 H2O
(20mM) |
10.00 | g | 10.582 | 42.933 | |
|
CaCl2 x 2 H2O
(30mM) |
10.00 | g | 10.582 | 71.979 | |
|
(NH4)2HPO4
(20mM) |
10.00 | g | 10.582 | 80.13 | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Distilled water | 940.00 | ml | - | - | |
| Vitamin solution | 3.00 | ml | - | - | |
| Glucose | 2.00 | mM | 0.36 | 2 | |
| 1 Adjust to pH 5.5 with NaOH/KOH (about 6 ml). | |||||
| 2 Autoclave and add 3 ml/l vitamin solution (see Medium1266) and 2mM Glucose end-concentration. | |||||
Media with this solution
| 1383 | VL55 MEDIUM modified |