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Modified Hutner`s Basal Salts Solution

Internal Solution-ID: S2891

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 10.00 g 9.524 49.826
MgSO4 x 7 H2O 29.70 g 28.286 114.761
CaCl2 x 2 H2O 3.34 g 3.181 21.637
Ammonium Molybdate 9.25 mg 0.009 0.045
FeSO4 x 7 H2O 99.00 mg 0.094 0.339
Metals "44" 50.00 ml - -
Distilled water 950.00 ml - -
1 Note: Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6 - 6.8 before bringing the volume to 1.0 L with distilled water.
1417 MMB MEDIUM