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Main sol. 1426

Internal Solution-ID: S2920

Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.50 g 0.5 -
Yeast extract 0.25 g 0.25 -
Glucose 0.10 g 0.1 0.555
MES
(10 mM final concentration)
1.95 g 1.95 9.988
SSE (double concentrated) 500.00 ml - -
Distilled water 500.00 ml - -
1 Add to 1000 ml of medium after autoclaving:
Trace element solution SL-10 1.00 ml - -
Vitamin solution 1.00 ml - -
2 adjust pH to 5.5
3 For solid medium use purified agar (Oxoid LP0028)
1426 SSE/HD 1:10 MEDIUM