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Solution 1

Internal Solution-ID: S2959

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCO3 5.0 g 5.208 52.037
Na2HPO4 x H2O 0.1 g 0.104 0.734
MgSO4 x 7 H2O 0.5 g 0.521 2.113
Hutner's salts 20.0 ml - -
Gelrite 8.0 g 9.375 -
Distilled water 930.0 ml - -
1 Adjust pH to 9.0, autoclave at 121°C for 20 min.
1441 ALKALIPHILES PIRELLULA MEDIUM (M1)