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Main sol. 1458

Internal Solution-ID: S2997

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 2.00 g 2 23.531
K2HPO4 0.50 g 0.5 2.871
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x 5 H2O 0.02 g 0.02 0.083
FeSO4 x 7 H2O 0.02 g 0.02 0.072
CaCl2 x 7 H2O 0.02 g 0.02 0.18
Glucose 5.00 g 5 27.753
Distilled water 1000.00 ml - -
Agar , for solid medium 15.00 g 15 44.599
1 Autoclave glucose separately. Adjust to pH 7.0
1458 Glucose minerals salts medium