Main sol. 1465
Internal Solution-ID: S3008
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 8.0 | g | 8 | 95.23 | |
| Na2CO3 | 22.0 | g | 22 | 207.571 | |
| NaCl | 6.0 | g | 6 | 102.669 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 10.0 with NaOH or HCl. Make anoxic under nitrogen stream and autoclave at 121°C. | |||||
| 2 After autoclaving add sterile, anoxic solutions of: | |||||
|
Chitin
, adjust in range 1 - 2 g/l if required
(amorphous, from 5% solution) |
1.5 | g | 1.5 | - | |
| MgSO4 | 1.0 | mM | 0.12 | 1 | |
| Yeast extract | 20.0 | mg | 0.02 | - | |
| NH4Cl | 4.0 | mM | 0.214 | 4 | |
| Na2S | 1.0 | mM | 0.079 | 1 | |
| Acidic trace metals (Pfennig & Lippert, 1965) | 1.0 | ml | - | - | |
| Vitamine mixture (Pfennig & Lippert, 1965) | 1.0 | ml | - | - | |
Media with this solution
| 1465 | CHITINISPIRILLUM MEDIUM |