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Main sol. 1485

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Internal Solution-ID: S3038

Equipment needed: Hungate tubes Gassing station Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 950 ml - -
Solution B 1 ml - -
Solution C 1 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
Solution H 10 ml - -
1 Dissolve ingredients of solution A except carbonates, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate, adjust pH to 9.0, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions B to H prepared under 100% N2 gas atmosphere. Adjust pH of the complete medium to 8.7 - 9.0, if necessary.
1485 DESULFONATRONUM ALKALITOLERANS MEDIUM