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1485. DESULFONATRONUM ALKALITOLERANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 950 ml - -
2 Solution B 1 ml - -
3 Solution C 1 ml - -
4 Solution D 10 ml - -
5 Solution E 10 ml - -
6 Solution F 10 ml - -
7 Solution G 10 ml - -
8 Solution H 10 ml - -
Instructions:
  1. Dissolve ingredients of solution A except carbonates, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate, adjust pH to 9.0, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
  2. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions B to H prepared under 100% N2 gas atmosphere. Adjust pH of the complete medium to 8.7 - 9.0, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 6.0 g 6.316 108.068
2 1.0 g 1.053 6.043
3 32.0 g 33.684 400.971
4 10.6 g 11.158 105.275
5 2.8 g 2.947 20.75
6 0.2 g 0.211 2.566
7 950.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace elements solution (Pfennig & Lippert,1966) 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 20 373.893
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 20 98.375
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20 mg 2 -
2 10 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.4 g 340 4999.47
2 10.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.9
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1008.27 55967.4
NaHCO3 32.258 383.994
Na2CO3 10.686 100.818
NaCl 6.048 103.493
Sodium formate 3.427 50.398
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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