1485 .
DESULFONATRONUM ALKALITOLERANS MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
8.7 - 9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Solution A | 950 | ml | ||
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Solution B | 1 | ml | ||
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Solution C | 1 | ml | ||
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Solution D | 10 | ml | ||
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Solution E | 10 | ml | ||
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Solution F | 10 | ml | ||
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Solution G | 10 | ml | ||
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Solution H | 10 | ml | ||
| 1 Dissolve ingredients of solution A except carbonates, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate, adjust pH to 9.0, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions B to H prepared under 100% N2 gas atmosphere. Adjust pH of the complete medium to 8.7 - 9.0, if necessary. | |||||
Solution A #
950
| Compound | Amount | Unit | |
|---|---|---|---|
|
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NaCl | 6.0 | g |
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K2HPO4 | 1.0 | g |
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NaHCO3 | 32.0 | g |
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Na2CO3 | 10.6 | g |
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Na2SO4 | 2.8 | g |
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Na-acetate | 0.2 | g |
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Distilled water | 950.0 | ml |
Trace elements solution (Pfennig & Lippert,1966) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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EDTA | 5.00 | g | ||
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FeSO4 x 7 H2O | 2.20 | g | ||
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ZnSO4 x 7 H2O | 0.10 | g | ||
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MnCl2 x 4 H2O | 0.03 | g | ||
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H3BO3 | 0.03 | g | ||
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CoCl2 x 6 H2O | 0.20 | g | ||
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CuCl2 x 2 H2O | 0.03 | g | ||
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NiCl2 x 6 H2O | 0.03 | g | ||
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Na2MoO4 x 2 H2O | 0.03 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 pH 3.0-4.0 | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
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NaOH | 0.5 | g |
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Na2SeO3 x 5 H2O | 3.0 | mg |
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Na2WO4 x 2 H2O | 4.0 | mg |
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Distilled water | 1000.0 | ml |