Main sol. 172a
Internal Solution-ID: S304
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.06 | g | 0.06 | 0.408 | |
| NaCl | 30.00 | g | 30 | 513.347 | |
| FeCl3 x 6 H2O | 1.25 | mg | 0.001 | 0.005 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Agar | 1.00 | g | 1 | 2.973 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0 and autoclave. | |||||
| 2 After autoclaving, add from separate sterile solutions per liter medium: | |||||
|
Glucose
(10% w/v) |
10.00 | ml | 1 | 5.551 | |
|
NaHCO3
(5% w/v) |
10.00 | ml | 0.5 | 5.952 | |
|
Na2S x 9 H2O
(3% w/v) |
3.30 | ml | 0.099 | 0.412 | |
| 3 Aseptically fill sterile 10-ml screwcap tubes almost completely with the medium. Make stab inoculation. | |||||
Media with this solution
| 172a | CY S2 MEDIUM |