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Solution D

Internal Solution-ID: S3068

Compound Amount Unit Conc. [g/L] Conc. [mM]
Pyridoxal hydrochloride 10.0 mg 0.1 0.491
Pyridoxal phosphate 10.0 mg 0.1 0.405
D , L-lactic acid, sodium salt 1120.0 mg 11.2 97.318
Hypoxanthine 2.8 mg 0.028 0.206
Uracil 2.2 mg 0.022 0.196
Thymidine 4.8 mg 0.048 0.198
N-(2-acetamido)-2-aminoethanesulfonic acid 3.6 mg 0.036 0.198
DL-carnitine 400.0 mg 4 24.814
Putrescine 2 HCl 10.0 mg 0.1 -
Phenol red , sodium salt 20.0 mg 0.2 0.564
Distilled water 100.0 ml - -
1 Prepare Solutions D, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
1494 . OMIZ-PAT Medium (modified)