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Solution L

Internal Solution-ID: S3076

Equipment needed: Filter Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(fresh)
42 g 300 -
Distilled water 140 ml - -
1 Homogenize 42 g fresh baker yeast in 140 ml distilled water, incubate for 24h at 56°C and centrifuge to harvest the supernatant. Adjust pH to 7 with NaOH, make anoxic, filter-sterilize and store at -80°C
1494 . OMIZ-PAT Medium (modified)