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Supplement 2

Internal Solution-ID: S3081

Compound Amount Unit Conc. [g/L] Conc. [mM]
Ascorbic acid 10 %(w/v) 100 567.795
Distilled water 2 ml - -
1 Ascorbic acid (10%; w/v); in distilled water, neutralize with NaOH
2 Prepare Supplements 2, flush with nitrogen to make them anoxic, filter-sterilize and store at -20°C
1494 . OMIZ-PAT Medium (modified)