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Mineral Salts Solution for solid Medium

Internal Solution-ID: S3091

Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Glutamic acid 1.0 g 2 13.593
Trisodium citrate x 2 H2O 1.0 g 2 6.8
KH2PO4 0.5 g 1 7.348
NH4Cl 0.5 g 1 18.695
K2SO4 2.5 g 5 28.693
MgCl2 x 7 H2O 20.0 g 40 420.122
Casamino acids 2.0 g 4 -
Yeast extract
(Oxoid)
1.0 g 2 -
FeSO4 2.0 mg 0.004 0.026
Trace Element Solution 1.0 ml - -
Distilled water 300.0 ml - -
NaCl 100.0 g 200 3422.31
Agar 20.0 g 40 118.931
Distilled water 200.0 ml - -
1497 HALOARCHAEUM SALINUM MEDIUM