Main sol. 1501
Internal Solution-ID: S3105
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution 1 (10x NMS salts) | 100.0 | ml | - | - | |
|
NaCl
(2 %) |
900.0 | ml | 18 | 308.008 | |
| Solution 2 (Fe EDTA) | 0.1 | ml | - | - | |
| Solution 3 (Sodium molybdate) | 1.0 | ml | - | - | |
| Trace elements | 1.0 | ml | - | - | |
| Agar , for solid medium | 1.5 | % | 15 | 44.599 | |
| Phosphate buffer | 10.0 | ml | - | - | |
| 1 Prepare the growth medium as follows: | |||||
| 2 Dilute 100 ml solution 1 to 1 litre with 2% NaCl and then add 1 ml of solution 3, 1 ml of the trace elements, and 0.1 ml of solution 2. If solid media is required add 1.5% agar. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 25% methane to the gas phase and autoclave at 121C (15psi) for 15 minutes. Autoclave the phosphate buffer separately. When the growth medium is cool 10 ml/l of the phosphate buffer is added - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking. | |||||
Media with this solution
| 1501 | METHYLOCALDUM MARINUM MEDIUM |