Main sol. 1521
Internal Solution-ID: S3148
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| NaCl | 20.00 | g | 20 | 342.231 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| Sodium citrate | 0.30 | g | 0.3 | 1.162 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| Ammonium acetate | 1.00 | g | 1 | 12.974 | |
| Lactate | 1.00 | g | 1 | 11.227 | |
| Pyruvate | 1.00 | g | 1 | 11.488 | |
| SL10 | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add distilled water to 1 liter, boil the medium and cool under N2, distribute under N2 and autoclave. After autoclaving the following sterilized solutions are added: | |||||
|
NaHCO3
(10%) |
10.00 | ml | 1 | 11.904 | |
|
Na2S x 9 H2O
(1 M) |
10.00 | ml | 2.402 | 10 | |
| Vitamin B12 | 10.00 | ml | - | - | |
Media with this solution
| 1521 | ARCOBACTER ANAEROPHILUS MEDIUM |