Main sol. 1559
Internal Solution-ID: S3218
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto Tryptone | 3.00 | g | 3 | - | |
|
Starch
(soluble) |
2.00 | g | 2 | - | |
| HEPES | 2.38 | g | 2.38 | 9.987 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| Vitamin B12 | 0.50 | mg | 5.0e-4 | 3.7e-4 | |
|
Agar
, for solid medium
(Difco) |
15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Sterilize vitamin B12 separately by filtration. Adjust pH of medium to 7.0 with KOH prior to autoclaving. | |||||
Media with this solution
| 1559 | TRYPTONE-STARCH MEDIUM |