Main sol. 1578
Internal Solution-ID: S3265
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.05 | g | 0.05 | - | |
|
Proteose peptone
(Difco no. 3) |
0.05 | g | 0.05 | - | |
| Casamino acids | 0.05 | g | 0.05 | - | |
| Glucose | 0.05 | g | 0.05 | 0.278 | |
|
Starch
(soluble) |
0.05 | g | 0.05 | - | |
| Na-pyruvate | 0.03 | g | 0.03 | 0.273 | |
| K2HPO4 | 0.03 | g | 0.03 | 0.172 | |
| MgSO4 x 7 H2O | 5.00 | mg | 0.005 | 0.02 | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Media with this solution
| 1578 | 1/10 R2A MEDIUM |