Main sol. 1579
Internal Solution-ID: S3266
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.33 | g | 0.329 | 6.151 | |
| KCl | 0.33 | g | 0.329 | 4.413 | |
| KH2PO4 | 0.33 | g | 0.329 | 2.418 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.329 | 1.618 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.329 | 2.238 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.997 | - | |
| KNO3 | 1.00 | g | 0.997 | 9.861 | |
| NaHCO3 | 2.00 | g | 1.994 | 23.736 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except yeast extract, nitrate, bicarbonate and vitamins) and sparge medium with 100% CO2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add yeast extract, nitrate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5 - 6.8, if necessary. | |||||
Media with this solution
| 1579 | PYROBACULUM FERRIREDUCENS MEDIUM |