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Main sol. 1579

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Internal Solution-ID: S3266

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.329 6.151
KCl 0.33 g 0.329 4.413
KH2PO4 0.33 g 0.329 2.418
MgCl2 x 6 H2O 0.33 g 0.329 1.618
CaCl2 x 2 H2O 0.33 g 0.329 2.238
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 1.00 g 0.997 -
KNO3 1.00 g 0.997 9.861
NaHCO3 2.00 g 1.994 23.736
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except yeast extract, nitrate, bicarbonate and vitamins) and sparge medium with 100% CO2 gas for at least 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add yeast extract, nitrate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be adjusted to 6.5 - 6.8, if necessary.
1579 PYROBACULUM FERRIREDUCENS MEDIUM