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Main sol. 1583

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Internal Solution-ID: S3271

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 535 mg 0.535 10.002
KH2PO4 54 mg 0.054 0.397
KCl 74 mg 0.074 0.993
MgSO4 x 7 H2O 49 mg 0.049 0.199
CaCl2 x 2 H2O 147 mg 0.147 1
NaCl 584 mg 0.584 9.993
Trace element solution 1 ml - -
Cresol red solution 0.5 g/L 2 ml - -
Distilled water 1000 ml - -
1 Autoclave, subsequently adjust pH of the medium to about 7.8 by addition of sterile 10% NaHCO3 (medium is turning from yellow to pink).
2 Important: During growth, add NaHCO3 to readjust pH as soon as pH indicator changes to yellow. For stock cultures, 5 g l-1 CaCO3 (will stay as a precipitate) or 4.8 g l-1 HEPES may be added to keep pH neutral for a longer time.
3 Aeration must be adjusted according to the density and activity of the culture. At low cell concentrations (which is the case most of the time) cells are sensitive to strong aeration (don't shake the cultures).
1583 MEDIUM FOR AMMONIA OXIDIZING BACTERIA (STRAINS FROM SOIL)