Main sol. 1588
Internal Solution-ID: S3282
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 10 | g | 10 | - | |
| Meat extract | 3 | g | 3 | - | |
| NaCl | 5 | g | 5 | 85.558 | |
| D(+)-Trehalose | 1 | g | 1 | 2.921 | |
| Agar , for solid medium | 19 | g | 19 | 56.492 | |
| Tap water | 250 | ml | - | - | |
| Natural sea water | 750 | ml | - | - | |
| 1 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water: | |||||
Media with this solution
| 1588 | BRADYMONAS SEDIMINIS MEDIUM |