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Main sol. 1647

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Internal Solution-ID: S3408

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.20 g 0.2 -
Glucose 0.18 g 0.18 0.999
KH2PO4 0.14 g 0.14 1.029
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.15 g 0.15 1.02
NH4Cl 0.54 ml 0.825 15.42
L-Cysteine HCl x H2O 0.30 g 0.3 1.708
NaHCO3 2.50 g 2.5 29.759
Na2S x 9 H2O 0.30 g 0.3 1.249
Resazurin 1.00 mg 1.0e-3 0.004
Vitamin solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 The vitamin solution, trace element solution, Se/W solution, cysteine, glucose, Na2S and NaHCO3 are added after the medium has been autoclaved.
1647 ACETOBACTEROIDES MEDIUM