Main sol. 1655
Internal Solution-ID: S3432
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 2.5 | g | 2.5 | - | |
| Tryptone | 2.5 | g | 2.5 | 35.172 | |
| Agar | 28.0 | g | 28 | 83.252 | |
| Nitrilotriacetic acid | 100.0 | mg | 0.1 | 0.523 | |
| CaSO4 x 2 H2O | 40.0 | mg | 0.04 | 0.232 | |
| MgCl2 x 6 H2O | 200.0 | mg | 0.2 | 0.984 | |
|
Fe(III) citrate
(0.01 M) |
0.5 | ml | 0.001 | 0.005 | |
| Trace element solution | 0.5 | ml | - | - | |
| Phosphate buffer | 100.0 | ml | - | - | |
| Distilled water | 900.0 | ml | - | - | |
| 1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. | |||||
| 2 Autoclave the phosphate buffer separately and then add to the medium. | |||||
Media with this solution
| 1655 | MODIFIED THERMUS MEDIUM 878 |