Main sol. 1668
Internal Solution-ID: S3459
Equipment needed: Hungate tubes
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.5 | g | 1 | - | |
| Tryptone | 0.5 | g | 1 | 14.069 | |
| Stock A: Salt solution 2x (50 ml stock per 100 ml medium) | 250.0 | ml | - | - | |
| Stock B: Salt solution 200x (0,5 ml stock per 100 ml medium) | 2.5 | ml | - | - | |
| Distilled water | 250.0 | ml | - | - | |
| L-Cysteine HCl | 1.0 | g/l | 1 | 6.344 | |
| 1 To make medium anoxic boil it, cool under N2CO2 gas atmosphere, add 1 g/l L-cysteine hydrochloride and adjust pH to 6.8. Finally gas over with N2 . Dispense under N2CO2 into suitable vessels for anaerobic growth (serum bottles or Hungate tubes sealed with rubber stoppers) and sterilize by autoclaving. | |||||
Media with this solution
| 1668 | MDM (modified dehority medium) |