MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1714

Download this solution as

CSV JSON PDF

Internal Solution-ID: S3551

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.20 g 0.198 1.497
KH2PO4 3.00 g 2.967 21.805
Allen's trace element solution 10.00 ml - -
Na2S2O3 x 5 H2O 2.50 g 2.473 9.963
MgSO4 x 7 H2O 0.50 g 0.495 2.007
CaCl2 x 2 H2O 0.25 g 0.247 1.682
Yeast extract
(OXOID) 		0.50 g 0.495 -
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.05 g 0.049 0.282
FeCl2 x 4 H2O 0.25 g 0.247 1.244
Distilled water 1000.00 ml - -
1 Dissolve ammonium sulfate, potassium phosphate and trace elements, adjust pH to 5.2 using 10 N H2SO4 and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium sulfate, calcium chloride, yeast extract, vitamins (sterilized by filtration), cysteine and ferrous chloride from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 5.0 - 5.2, if necessary.
1714 CONEXIVISPHAERA MEDIUM