Main sol. J21
Internal Solution-ID: S3644
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone , tryptic digest | 10.0 | g | 9.606 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 4.803 | - | |
|
Beef extract
(BD-Difco) |
2.0 | g | 1.921 | - | |
| Glucose | 20.0 | g | 19.212 | 106.64 | |
| KH2PO4 | 0.5 | g | 0.48 | 3.529 | |
| K2HPO4 | 0.5 | g | 0.48 | 2.758 | |
| FeSO4 x 7 H2O | 10.0 | mg | 0.01 | 0.035 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.192 | 0.779 | |
| MnSO4 x n H2O | 7.5 | mg | 0.007 | - | |
| Tween 80 | 1.0 | ml | - | - | |
| Ethanol | 40.0 | ml | - | - | |
| Sodium acetate | 20.0 | g | 19.212 | 234.211 | |
| DL-mevalonic acid | 30.0 | mg | 0.029 | 0.195 | |
| Agar | 15.0 | g | 14.409 | 42.843 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 5.2. | |||||
Media with this solution
| J21 | LACTOBACILLUS MEDIUM III |