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Soil extract

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Internal Solution-ID: S3735

This solution is used in 3 media and was first defined in medium J102.

Equipment needed: Autoclave
1 Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation.
J102 NUTRIENT AGAR WITH 25% SOIL EXTRACT
J342 BRAIN HEART INFUSION AGAR WITH SOIL EXTRACT
J407 NUTRIENT AGAR WITH 50% SOIL EXTRACT AND 5% NaCl