J102
102 .
NUTRIENT AGAR WITH 25% SOIL EXTRACT
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J102 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Beef extract | 3 | g | 3 | - | |
| Soil extract | 250 | ml | - | - | |
| Agar | 15 | g | 15 | 44.599 | |
| Tap water | 750 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
Solution occurs in 2
other media (first defined in Medium J102).
Equipment needed: Autoclave
| 1 Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation. |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |