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Main sol. J386

Internal Solution-ID: S4113

Compound Amount Unit Conc. [g/L] Conc. [mM]
Middlebrook 7H10 agar
(BD-Difco)
19 g 21.111 -
Glycerol 5 ml - -
Distilled water 895 ml - -
1 Sterilize at 121C for 10 min and cool to 50 - 55C. Aseptically add 100 ml of Middlebrook OADC Enrichment (BD-BBL) and mix thoroughly.
J386 MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT