Main sol. J431
Internal Solution-ID: S4169
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Basal medium | 1000.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Vitamin B12 | 5.00 | �g | - | - | |
| Glucose | 3.60 | g | 3.557 | 19.745 | |
|
Yeast extract
(BD-Difco) |
0.50 | g | 0.494 | - | |
|
Tryptone
(BD-Difco) |
1.50 | g | 1.482 | 20.853 | |
|
Sulfur
(powder) |
20.00 | g | 19.763 | 616.241 | |
| NaHCO3 | 3.40 | g | 3.36 | 39.993 | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Na2S x 9 H2O | 0.36 | g | 0.356 | 1.481 | |
| 1 Autoclave Basal medium supplemented with Trace element solution, Selenite-tungstate solution and resazurin under N2-CO2 (4:1, v/v). Separately autoclave glucose, yeast extract and tryptone (10% each) and add to the medium. Filter-sterilize Trace vitamins and NaHCO3 (8%) solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na2S x 9H2O solution (autoclaved under a N2 atmosphere). Readjust pH to 7.2, if necessary. | |||||
Media with this solution
| J431 | BN3 MEDIUM |