Main sol. J459
Internal Solution-ID: S4206
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD-BBL) |
17 | g | 17 | - | |
|
Yeast extract
(BD-Difco) |
3 | g | 3 | - | |
| Glucose | 3 | g | 3 | 16.652 | |
| NaCl | 3 | g | 3 | 51.335 | |
| KNO3 | 3 | g | 3 | 29.673 | |
| Hemin | 3 | mg | 0.003 | 0.005 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Autoclave glucose solution separately. Incubate under an oxygen-free atmosphere containing 5-10% CO2. | |||||
Media with this solution
| J459 | CAPNOCYTOPHAGA MEDIUM |