Main sol. J545
Internal Solution-ID: S4353
Equipment needed: Autoclave
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.297 | 2.183 | |
| K2HPO4 | 0.3 | g | 0.297 | 1.705 | |
| NH4Cl | 1.0 | g | 0.99 | 18.51 | |
| NaCl | 1.0 | g | 0.99 | 16.942 | |
| Na2SO4 | 3.0 | g | 2.97 | 20.912 | |
| KCl | 0.1 | g | 0.099 | 1.328 | |
| CaCl2 | 0.1 | g | 0.099 | 0.892 | |
| MgCl2 x 6 H2O | 0.5 | g | 0.495 | 2.435 | |
| Yeast extract | 0.1 | g | 0.099 | - | |
| Sodium lactate | 1.0 | g | 0.99 | 8.835 | |
| Trace element solution | 10.0 | ml | - | - | |
| NaHCO3 | 2.0 | g | 1.98 | 23.572 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.495 | - | |
| Na2S x 9 H2O | 0.4 | g | 0.396 | 1.649 | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a N2 x CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO3 [5%, filter-sterilized and stored under a N2-CO2 (4:1, v/v) gas mixture], L-cysteine x HCl x H2O and Na2S x 9H2O (5%, autoclaved and stored under N2) to the medium. | |||||
Media with this solution
| J545 | DESULFOTOMACULUM Lam5 MEDIUM |