Solution A
Internal Solution-ID: S4356
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 2.0 | g | 2.02 | - | |
| Beef extract | 1.0 | g | 1.01 | - | |
| MgSO4 x 7 H2O | 2.0 | g | 2.02 | 8.196 | |
| Na2SO4 | 1.5 | g | 1.515 | 10.667 | |
| CaCl2 | 0.1 | g | 0.101 | 0.91 | |
| Distilled water | 900.0 | ml | - | - | |
| 1 To complete the medium, aseptically and anaerobically add the following solutions to the medium: | |||||
|
K2HPO4
(autoclaved, 5%) |
10.0 | ml | - | - | |
|
Sodium lactate
(autoclaved, 5%) |
70.0 | ml | - | - | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(filter-sterilized, 4%) |
10.0 | ml | - | - | |
|
Sodium ascorbate
(filter-sterilized, 1%) |
10.0 | ml] | - | - | |
| 2 Mix components and autoclave under a N2 gas atmosphere. | |||||
Media with this solution
| J547 | DESULFOTOMACULUM PBE MEDIUM |