Main sol. J574
Internal Solution-ID: S4402
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MDS salt water | 833.00 | ml | - | - | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
|
Peptone
(Oxoid) |
0.25 | g | 0.25 | - | |
|
Yeast extract
(BD-Difco) |
0.05 | g | 0.05 | - | |
| 1 Add components and bring volume to 980 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar as described below). Autoclave, and after cooling, add the following solutions (filter-sterilized or *autoclaved) to the medium: | |||||
|
NH4Cl
(1 M) |
5.00 | ml | 0.267 | 5 | |
|
Potassium phosphate buffer
(*) |
2.00 | ml | - | - | |
| Vitamin solution | 3.00 | ml | - | - | |
|
Sodium pyruvate
(1 M) |
10.00 | ml | 1.1 | 10 | |
| 2 Readjust pH to 7.0 - 7.5 with a sterilized 10% Na2CO3 solution, if necessary. | |||||
Media with this solution
| J574 | DB CHARACTERIZATION MEDIUM NO. 2 |