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Substrate

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Internal Solution-ID: S4410

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium palmitate 2.8 g - -
1 Autoclave in culture vessels.
2 To complete the medium, combine Solution A and substrate in the culture vessels under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally, reduce the medium with 0.01 volume of 5% Na2S x 9H2O solution (autoclaved and stored under a N2 atmosphere).
J576 DESULFATIBACILLUS OLEFINIVORANS MEDIUM