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Main sol. J580

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Internal Solution-ID: S4417

1 Add the following component after autoclaving:
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.499 3.667
MgCl2 x 6 H2O 0.20 g 0.2 0.982
NaCl 0.40 g 0.399 6.831
NH4Cl 1.20 g 1.198 22.389
CaCl2 x 2 H2O 0.05 g 0.05 0.339
Sorbitol 3.00 g 2.994 16.435
Sodium pyruvate 3.00 g 2.994 27.208
Yeast extract 0.40 g 0.399 -
Distilled water 1000.00 ml - -
Micronutrient solution SL7 1.00 ml - -
Vitamin B12
(20 �g/L) 		1.00 ml - -
2 Adjust pH to 7.0.
J580 RHODOBACTER SPHAEROIDES MEDIUM