Solution A
Internal Solution-ID: S4437
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 19.493 | 333.559 | |
| K2HPO4 | 0.09 | g | 0.088 | 0.504 | |
| KH2PO4 | 0.07 | g | 0.068 | 0.501 | |
| CaCl2 x 2 H2O | 0.80 | g | 0.78 | 5.304 | |
| NH4Cl | 1.00 | g | 0.975 | 18.221 | |
| NaNO3 | 1.00 | g | 0.975 | 11.467 | |
| MgSO4 x 7 H2O | 1.00 | g | 0.975 | 3.954 | |
| MgCl2 x 6 H2O | 6.00 | g | 5.848 | 28.765 | |
| KCl | 0.33 | g | 0.322 | 4.314 | |
| NiCl2 x 6 H2O | 1.00 | mg | 9.7e-4 | 0.008 | |
| Na2SeO3 x 5 H2O | 1.00 | mg | 9.7e-4 | 0.004 | |
| Fe2(SO4)3 x n H2O | 0.01 | g | 0.01 | - | |
| H2WO4 | 1.00 | mg | 9.7e-4 | 0.004 | |
| Na2S2O3 x 5 H2O | 3.00 | g | 2.924 | 11.781 | |
| FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.035 | |
| Ferrous citrate | 0.01 | g | 0.01 | - | |
| Trace mineral solution | 5.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients and adjust pH to 6.8 with NaOH. Distribute the solution into culture vessels (leaving more than 80% volume of gas phase in the vessel) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Then, add the following solutions (filter-sterilized) to the medium (per liter): | |||||
| Trace vitamins | 1.00 | ml | - | - | |
|
NaHCO3
(5%) |
20.00 | ml | - | - | |
| 2 Finally, replace the gas phase with a N2-CO2-O2 (79:20:1, by volume) gas mixture and pressurize to 200 kPa with the same gas mixture. | |||||
Media with this solution
| J592 | MODIFIED MMJS MEDIUM (B) |